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Chemical Decontamination of iPS Cell-Derived Neural Cell Mixtures

Abstract

The tumorigenic risk of residual stem cells is one of the major hurdles in stem cell therapy. The potential tumor risk is particularly relevant to transplantation of neurons or neuronal precursors derived from human induced pluripotent stem cells (hiPSCs). This report describes the design and evaluation of phosphorylated 7-Ethyl-10-hydroxycamptothecin (SN38-P), which selectively eliminates tumor-forming proliferative stem cells, including human iPS and neural stem cells, from iPSC-derived neural cell mixtures. The selectivity of SN38-P takes advantage of the high expression level of alkaline phosphatase on the proliferative stem cells. Results of the present study demonstrate that simple phosphorylation of an anticancer drug can provide a safe, cost-effective, and chemically-defined tool for decontaminating hiPSC-derived neuron.

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Publication details

The article was received on 13 Nov 2017, accepted on 12 Jan 2018 and first published on 12 Jan 2018


Article type: Communication
DOI: 10.1039/C7CC08686E
Citation: Chem. Commun., 2018, Accepted Manuscript
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    Chemical Decontamination of iPS Cell-Derived Neural Cell Mixtures

    D. Mao, X. K. W. Chung, T. Andoh-Noda, Y. Qin, S. Sato, Y. Takemoto, W. Akamatsu, H. Okano and M. Uesugi, Chem. Commun., 2018, Accepted Manuscript , DOI: 10.1039/C7CC08686E

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