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Issue 15, 2018
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Enzymatically cleavable traceless biotin tags for protein PEGylation and purification

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Abstract

Here we report an example of a protein–PEG conjugate with a biotin tag cleavable by lipase-catalyzed hydrolysis. Very mild cleavage conditions, heterogeneous, easily separable catalysts, and traceless design make this method attractive for the preparation and purification of PEGylated proteins.

Graphical abstract: Enzymatically cleavable traceless biotin tags for protein PEGylation and purification

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Publication details

The article was received on 26 Jul 2017, accepted on 05 Jan 2018 and first published on 08 Jan 2018


Article type: Communication
DOI: 10.1039/C7CC05814D
Citation: Chem. Commun., 2018,54, 1913-1916
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    Enzymatically cleavable traceless biotin tags for protein PEGylation and purification

    A. M. Wawro, Y. Aoki, T. Muraoka, K. Tsumoto and K. Kinbara, Chem. Commun., 2018, 54, 1913
    DOI: 10.1039/C7CC05814D

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