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Impact of substrate stiffness on dermal papilla aggregates in microgels

Abstract

Interaction between cells and extracellular environment plays a vital role in cellular development. The mechanical property of a 3-dimensional (3D) culture can be modified to mimic in-vivo conditions. Dermal Papilla (DP) cells are shown to gradually lose their inductivity in the hair cycle development in 2-dimensional culture. They are shown to partially restore that inductivity when transferred into a 3D microenvironment. In this study, a microarray fabricated from three different concentrations of poly-ethylene-glycol-diacrylate 3500, namely 5%, 10% and 15% w/w, yielded increasing substrate stiffness. The impact of varying substrate stiffness was tested for DP cell viability, attachment, and selected hair inductive markers. DP aggregates were shown to be viable and exhibited greater spreading with increasing substrate stiffness. Moreover, DP aggregates cultured on softer substrate showed greater fold change of gene and protein expressions than those cultured on a harder substrate.

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Publication details

The article was received on 02 Mar 2018, accepted on 28 Mar 2018 and first published on 29 Mar 2018


Article type: Paper
DOI: 10.1039/C8BM00248G
Citation: Biomater. Sci., 2018, Accepted Manuscript
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    Impact of substrate stiffness on dermal papilla aggregates in microgels

    J. Tan, J. K. Tee, K. O. Chou, S. Auyong, J. Pan, H. K. Ho, C. L. P. Ho and L. Kang, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C8BM00248G

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