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Scaffold-free cell culturing in three-dimension using magnetic levitation

Abstract

Three-dimensional (3D) cell culture has emerged as a pioneering methodology and has increasingly utilized for tissue engineering, 3D bioprinting, cancer model studies and drug development studies. 3D cell culture methodology provides artificial and functional cellular constructs serving as a modular playground prior to animal model studies that saves substantial efforts, time and experimental costs. The major drawback of current 3D cell culture methods is the dependency to the biocompatible scaffolds, which often requires tedious synthesis and fabrication steps. Here we report an easy-to-use direct forward methodology for formation of scaffold-free 3D cell culture and cellular assembly via magnetic levitation in the presence of paramagnetic agents. To paramagnetize the cell culture environment three different Gadolinium (III) chelates were utilized which lead levitation and assembly of cells at certain levitation height. The assembly and close interaction of cells at levitation height where the magnetic force was equilibrated with gravitational force, trigger the formation of complex 3D cellular structures. It is shown that Gd (III) chelates provided an optimal levitation that induces intercellular interactions in scaffold-free format without comprising cell viability. NIH 3T3 mouse fibroblast and HCC827 non-small-cell lung cancer cells were evaluated via magnetic levitation system and formation of 3D cell culture models have been validated for both cell lines. Hereby developed magnetic levitation system hold promises for complex cellular assemblies and 3D cell culture studies.

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Publication details

The article was received on 31 Jan 2018, accepted on 12 Apr 2018 and first published on 12 Apr 2018


Article type: Paper
DOI: 10.1039/C8BM00122G
Citation: Biomater. Sci., 2018, Accepted Manuscript
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    Scaffold-free cell culturing in three-dimension using magnetic levitation

    E. Turker, N. Demircak and A. Arslan Yildiz, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C8BM00122G

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