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Controlling the orientation of cell-synthesized extracellular matrix by using engineered gelatin-based building-blocks

Abstract

In tissue engineering there is a growing interest in fabricating highly engineered platforms designed to instruct cells towards the synthesis of tissues that reproduce their natural counterpart. In this context, a fundamental factor to take into account is the control over the final tissue orientation, especially for what concerns the replication of load-bearing tissues whose functions are strictly related to their microstructural organization. Starting from this point, in this work we have engineered a gelatin-based hydrogel in order to be patterned by 2-photon polymerization (2PP) lithography for the fabrication of instructive free standing building blocks designed to produce anisotropic collagen-based µtissues. Biological results clearly highlighted the strong relationship between µtissue orientation and such topographies, which resulted in fact a crucial element in the production of highly anisotropic µtissues.

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Publication details

The article was received on 24 Nov 2017, accepted on 31 May 2018 and first published on 04 Jun 2018


Article type: Paper
DOI: 10.1039/C7BM01093A
Citation: Biomater. Sci., 2018, Accepted Manuscript
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    Controlling the orientation of cell-synthesized extracellular matrix by using engineered gelatin-based building-blocks

    F. A. Pennacchio, C. Casale, F. Urciuolo, G. Imparato, R. Vecchione and P. A. Netti, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C7BM01093A

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