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Evaluation of RGD functionalization in hybrid hydrogels as 3D neural stem cell culture systems

Abstract

The use of neural stem cells (NSCs) in cell therapy appears as a powerful tool for the treatment of central nervous system diseases, including traumatic brain and spinal cord injuries. However, a significant drawback is related to the limited viability after transplantation in situ. The design of three-dimensional (3D) scaffolds able to resemble the architecture and the physico-chemical features of extracellular environment would be a suitable approach to improve cell survival and preserve their cellular active phase over time. In this study, we investigated NSC adhesion and proliferation in hydrogel systems; in particular, we evaluated the effect of RGD binding domains on cell fate within the polymeric scaffold. The introduction of the tripeptide, through hydrogel chemical functionalization, improved the percentage of proliferating cells until 8 days after the seeding than an unmodified scaffold. The benefic effect of this 3D culture system was further evident when compared to NSC monolayer (2D) culture, resulting approximately in 40% increase of cells in active phases at 4 and 8 days and maintaining a difference of 25% until 21 days after the seeding.

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Publication details

The article was received on 15 Nov 2017, accepted on 08 Jan 2018 and first published on 09 Jan 2018


Article type: Paper
DOI: 10.1039/C7BM01056G
Citation: Biomater. Sci., 2018, Accepted Manuscript
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    Evaluation of RGD functionalization in hybrid hydrogels as 3D neural stem cell culture systems

    E. Mauri, A. Sacchetti, N. Vicario, L. Peruzzotti-Jametti , F. Rossi and S. Pluchino, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C7BM01056G

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