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Enhanced bone defect repairing effects in glucocorticoids-induced osteonecrosis of femoral head using porous nano-lithium-hydroxyapatite/gelatin microspheres/ erythropoietin composite scaffold

Abstract

Glucocorticoids-induced osteonecrosis of femoral head (GIONFH) is a common debilitating disease that occurs in young and middle-aged adults. To treat the early GIONFH, core decompression and bone graft are regarded as effective measures. However, the ideal bone graft should possess bioactivity as well as biomechanical property. The commonly used bone graft materials are still unsatisfactory at nowadays. In this study, we fabricated a composited scaffold using lithium (Li) to activate the Wnt signal pathway and erythrogenin (EPO) to upregulate HIF-1/VEGF pathway to improve the osteogenic and angiogenic effects. We obtained the porous gelatin/ nano-lithium-hydroxyapatite/ gelatin microspheres/ rhEPO (Li-nHA/GMs/rhEPO) composited scaffold and assessed this scaffold’s mechanical property, release property and in vitro bioactivity. Then, we implanted the scaffold into the GIONFH rabbit femoral head after core decompression surgery and evaluated its osteogenic and angiogenic abilities in vivo as well as the bone defect repair efficacy. As the results shown, Li-nHA/GMs/rhEPO scaffold possessed good mechanical compression strength, and could control the release of Li and rhEPO continuously. Moreover, the scaffold could improve the viability of glucocorticoids treated BMMSCs and vascular endothelial cells, and increased the expression of osteogenic and angiogenic factors. In the vivo study, the composited scaffold improved the new bone formation and exerted effects on repairing the femoral head defects in GIONFH rabbit. Besides, the osteogenic and angiogenic factors were increased along with the activation of factors in Wnt signal pathway and HIF-1/VEGF pathway. In conclusion, the Li-nHA/GMs/rhEPO scaffold could upregulate the Wnt and HIF-1/VEGF pathway at same time and has effect on improving the osteogenesis and angiogenesis, which benefited to repair the GIONFH.

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Publication details

The article was received on 25 Oct 2017, accepted on 09 Jan 2018 and first published on 10 Jan 2018


Article type: Paper
DOI: 10.1039/C7BM00975E
Citation: Biomater. Sci., 2018, Accepted Manuscript
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    Enhanced bone defect repairing effects in glucocorticoids-induced osteonecrosis of femoral head using porous nano-lithium-hydroxyapatite/gelatin microspheres/ erythropoietin composite scaffold

    D. Li, X. Xie, Z. Yang, C. Wang, Z. Wei and P. Kang, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C7BM00975E

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