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Three-dimensional multiscale fiber matrices: Development and characterization for increased HepG2 functional maintenance for bio-artificial liver application

Abstract

The development of a cell-growth substrate that provides a natural-like microenvironment, promotes cell adhesion, and maintains cells functional activities, is a research focus in the field of tissue engineering. In the present study, three-dimensional micro-nano multiscale fiber-based substrates were developed by depositing biocompatible polycaprolactone (PCL)/PCL-Chitosan (C)/ PCL-C-Gelatin (G) electrospun nanofibers (NFs) on the outer surface of hollow fiber membranes (HFMs) in one step. A comparison study with regard to physico-chemical characterization, hemocompatibility, cytotoxicity, and cellular functionality was performed with the developed matrices. PCL-C-G NFs-deposited HFMs-based matrix showed superior hemocompatibility for blood-contact applications. Cytotoxicity of these matrices was found to be minimal. HepG2 cells exhibited exceptionally robust adherence and proliferated growth on the matrix with the formation of characteristic multi-cellular spheroids. Further, cell functional activities such as albumin secretion, urea synthesis, and cytochrome P450 specific activity were measured for the developed matrices. The developed three-dimensional multiscale fibers-based matrix can be a potential membrane for bioreactor and bio-artificial liver applications.

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Publication details

The article was received on 23 Oct 2017, accepted on 19 Dec 2017 and first published on 02 Jan 2018


Article type: Paper
DOI: 10.1039/C7BM00963A
Citation: Biomater. Sci., 2018, Accepted Manuscript
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    Three-dimensional multiscale fiber matrices: Development and characterization for increased HepG2 functional maintenance for bio-artificial liver application

    S. K. Verma, A. Modi and J. Bellare, Biomater. Sci., 2018, Accepted Manuscript , DOI: 10.1039/C7BM00963A

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