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Cells on hierarchically-structured platforms hosting functionalized nanoparticles

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Abstract

In this work, we report on a novel approach to develop hierarchically-structured cell culture platforms incorporating functionalized gold nanoparticles (AuNPs). In particular, the hierarchical substrates comprise primary pseudo-periodic arrays of silicon microcones combined with a secondary nanoscale pattern of homogeneously deposited AuNPs terminated with bio-functional moieties. AuNPs with various functionalities (i.e. oligopeptides, small molecules and oligomers) were successfully attached onto the microstructures. Experiments with PC12 cells on hierarchical substrates incorporating AuNPs carrying the RGD peptide showed an impressive growth and NGF-induced differentiation of the PC12 cells, compared to that on the NP-free, bare, micropatterned substrates. The exploitation of the developed methodology for the binding of AuNPs as carriers of specific bio-functional moieties onto micropatterned culture substrates for cell biology studies is envisaged.

Graphical abstract: Cells on hierarchically-structured platforms hosting functionalized nanoparticles

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Publication details

The article was received on 04 Oct 2017, accepted on 12 Mar 2018 and first published on 13 Mar 2018


Article type: Paper
DOI: 10.1039/C7BM00904F
Citation: Biomater. Sci., 2018, Advance Article
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    Cells on hierarchically-structured platforms hosting functionalized nanoparticles

    C. Simitzi, P. Harimech, S. Spanou, C. Lanara, A. Heuer-Jungemann, A. Manousaki, C. Fotakis, A. Ranella, A. G. Kanaras and E. Stratakis, Biomater. Sci., 2018, Advance Article , DOI: 10.1039/C7BM00904F

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