Jump to main content
Jump to site search


An engineered cell-imprinted substrate directs osteogenic differentiation in stem cells

Author affiliations

Abstract

A cell-imprinted poly(dimethylsiloxane)/hydroxyapatite nanocomposite substrate was fabricated to engage topographical, mechanical, and chemical signals to stimulate and boost stem cell osteogenic differentiation. The physicochemical properties of the fabricated substrates, with nanoscale resolution of osteoblast morphology, were probed using a wide range of techniques including scanning electron microscopy, atomic force microscopy, dynamic mechanical thermal analysis, and water contact angle measurements. The osteogenic differentiation capacity of the cultured stem cells on these substrates was probed by alizarin red staining, ALP activity, osteocalcin measurements, and gene expression analysis. The outcomes revealed that the concurrent roles of the surface patterns and viscoelastic properties of the substrate provide the capability of directing stem cell differentiation toward osteogenic phenotypes. Besides the physical and mechanical effects, we found that the chemical signaling of osteoinductive hydroxyapatite nanoparticles, embedded in the nanocomposite substrates, could further improve and optimize stem cell osteogenic differentiation.

Graphical abstract: An engineered cell-imprinted substrate directs osteogenic differentiation in stem cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 12 Aug 2017, accepted on 14 Nov 2017 and first published on 20 Nov 2017


Article type: Paper
DOI: 10.1039/C7BM00733G
Citation: Biomater. Sci., 2018, Advance Article
  •   Request permissions

    An engineered cell-imprinted substrate directs osteogenic differentiation in stem cells

    K. Kamguyan, A. A. Katbab, M. Mahmoudi, E. Thormann, S. Zajforoushan Moghaddam, L. Moradi and S. Bonakdar, Biomater. Sci., 2018, Advance Article , DOI: 10.1039/C7BM00733G

Search articles by author

Spotlight

Advertisements