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Simultaneous determination of aflatoxin B1 and zearalenone by magnetic nanoparticles filled amino-modified multi-walled carbon nanotubes

Abstract

A novel magnetic solid-phase extraction (MSPE) coupled with high performance liquid chromatography (HPLC) method was explored for aflatoxin B1 (AFB1) and zearalenone (ZON) simultaneous determination. The method was based on a new explored extractant of Fe3O4 nanoparticles filled amino-modified multi-walled carbon nanotubes (Fe3O4-MWCNTs-NH2) which was sensitive to analytes with the structure of multi-aromatic rings and more hydrogen bond acceptors based on the extraction mechanism of π-π EDA and hydrogen-bonding interactions. The morphology, structure and magnetic property of the extractant were characterized by transmission electron microscope (TEM), Fourier transform infrared (FTIR) spectra, X-ray diffraction (XRD) and vibrating sample magnetometer (VSM), respectively. The adsorption isotherms and kinetics for two mycotoxins were investigated and proved to be better fitting for Dubinin-Ashtakhov and pseudo-second-order models. The critical MSPE parameters including solution pH, extraction time, salt addition and temperature were investigated and optimized, and the established analytical method was validated. Subsequently the method was successfully applied for AFB1 and ZON analysis from wheat flour samples. The proposed method was proved to be an excellent green analysis with the score of 89 in analytical Eco-Scale evaluation, and have good linearities in the range of 1.0–100.0 ng/g with the linear coefficients (r) above 0.99, desirable recoveries ranged from 88.8% to 96.0%, satisfactory precision with the relative standard deviation (RSD) in the range of 2.1–2.8%, and low limits of detection (LODs) that were 0.15 and 0.24 ng/g for AFB1 and ZON, respectively. The novel magnetic extractant based green MSPE-HPLC method has great potentials for the mycotoxins analysis from complex matrix samples.

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Supplementary files

Publication details

The article was received on 11 Apr 2018, accepted on 12 Jun 2018 and first published on 13 Jun 2018


Article type: Paper
DOI: 10.1039/C8AY00815A
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Simultaneous determination of aflatoxin B1 and zearalenone by magnetic nanoparticles filled amino-modified multi-walled carbon nanotubes

    W. Li, H. Zhang and Y. Shi, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00815A

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