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Target Regulated Photo Induced Electron Transfer of DNA-Cu Nanoparticles and its application for the detection of Hepatitis B gene

Abstract

Despite of the distinct features of poly thymine (T)-templated copper nanoparticles (polyT-Cu NPs) as fluorescent probes for various biosensors, most of the reported methods usually required labeling with an appropriate fluorescent quencher, or the addition of enzyme to digest DNA-template. In this paper, it was first found that the phenomenon of photo induced electron transfer (PET) between the polyT-Cu NPs and the Guanine quadruplex (G4)-K+-hemin (Horseradish Peroxidase (HRP)-DNAzyme) complexes, which was further applied for the fabrication of a “turn-on” target modulated fluorescent system for sensitive determination of HBV gene without any labeling process or the addition of enzyme. By the optical and electrical experiments, the PET mechanism was investigated. In the detection process, the PET leaded the fluorescence of polyT-Cu NPs was quenched lowering the background, and then in the presence of target DNA, by the conformational change, the PET was blocked and the fluorescence could be recovered. The developed strategy was highly sensitive for the detection of HBV gene in the concentration ranging from 0.1 to 80 nM with a low detection limit of 33.4 pM (S/N = 3). In addition, the novel method for specific DNA sequence detection was highly selective. Finally, this work was successfully utilized for the sensitive and label free fluorescent determination of HBV gene in the blood plasma samples.

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Publication details

The article was received on 01 Apr 2018, accepted on 30 Apr 2018 and first published on 03 May 2018


Article type: Paper
DOI: 10.1039/C8AY00722E
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Target Regulated Photo Induced Electron Transfer of DNA-Cu Nanoparticles and its application for the detection of Hepatitis B gene

    Q. Xie, D. Shi, J. Wan, G. Wang and X. Zhang, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00722E

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