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Issue 23, 2018
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A colorimetric method for cysteine determination based on the peroxidase-like activity of ficin

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Abstract

The concentration of L-cysteine (L-Cys) provides important information about specific physiological functions and disease diagnosis. In this work, we provided a facile colorimetric approach for L-Cys detection with high sensitivity and specificity. Ficin acted as a peroxidase mimic and catalyzed the chromogenic reaction of TMB and H2O2. However, after the introduction of L-Cys, L-Cys competed with TMB for H2O2. The competition is generally won by the more-reactive L-Cys resulting in fading of the chromogenic reaction. The detection limit of L-Cys can be as low as 20 nM (3σ). In addition, the assay can also be successfully applied to detect L-Cys in biological fluids, indicating its potential in biological sample analysis. Our work could promote the application of the enzyme mimicking activity of ficin in medical diagnostics and biochemistry.

Graphical abstract: A colorimetric method for cysteine determination based on the peroxidase-like activity of ficin

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Publication details

The article was received on 30 Mar 2018, accepted on 08 May 2018 and first published on 10 May 2018


Article type: Paper
DOI: 10.1039/C8AY00707A
Citation: Anal. Methods, 2018,10, 2676-2680
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    A colorimetric method for cysteine determination based on the peroxidase-like activity of ficin

    Z. Huang, Y. Yang, Y. Long and H. Zheng, Anal. Methods, 2018, 10, 2676
    DOI: 10.1039/C8AY00707A

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