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A Colorimetric Method for Cysteine Determination based on the Peroxidase-like Activity of Ficin

Abstract

The concentration of L-cysteine (L-Cys) provides important information for specific physiological function and disease diagnosis. In this work, we provided a facile colorimetric approach for L-Cys detection with high sensitivity and specificity. Ficin acted as a peroxidase mimic and catalyzed the chromogenic reaction of TMB and H2O2. However, after the introduction of L-Cys, L-Cys competed with TMB for H2O2. The competition is generally won by the more-reactive L-Cys resulting in fading the chromogenic reaction. The detection limit of L-Cys can be as low as 20 nM (3σ). In addition, the assay can also be successfully applied to detect L-Cys in biological fluids, indicating its potential in biological sample analysis. Our work could promote the application of the enzymatic mimic activity of ficin in medical diagnostics and biochemistry.

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Publication details

The article was received on 30 Mar 2018, accepted on 08 May 2018 and first published on 10 May 2018


Article type: Paper
DOI: 10.1039/C8AY00707A
Citation: Anal. Methods, 2018, Accepted Manuscript
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    A Colorimetric Method for Cysteine Determination based on the Peroxidase-like Activity of Ficin

    Z. Huang, Y. Yang, Y. Long and H. Zheng, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00707A

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