Jump to main content
Jump to site search

Issue 26, 2018
Previous Article Next Article

A long-wavelength fluorescent probe for amino compounds and its application in the determination of aliphatic amines

Author affiliations

Abstract

Herein, a highly reactive long-wavelength fluorescent labeling reagent, 1,7-dimethyl-3,5-distyryl-8-phenyl-(4-oxy-acetic acid N-hydroxysuccinimidyl ester) difluoroboradiaza-s-indacene (DMDSPAB-OSu), was designed and synthesized for amino compounds. Using DMDSPAB-OSu as a pre-column derivatization reagent, an HPLC-fluorescence detection method was developed for the determination of trace amounts of aliphatic amines in samples. Prior to analysis, amines were derivatized with DMDSPAB-OSu at room temperature in 0.2 mol Lāˆ’1 pH 8.8 borate buffer for only 5 min, and the baseline separation of 11 aliphatic amines was achieved within 25 min. Limits of detection between 0.8 to 1.2 nM were obtained at 630 nm with an excitation wavelength of 610 nm. Furthermore, the proposed DMDSPAB-OSu-based HPLC method was applied to the analysis of lake water and human urine with satisfying recoveries ranging from 93.0ā€“109.7%.

Graphical abstract: A long-wavelength fluorescent probe for amino compounds and its application in the determination of aliphatic amines

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Apr 2018, accepted on 05 Jun 2018 and first published on 06 Jun 2018


Article type: Paper
DOI: 10.1039/C8AY00706C
Citation: Anal. Methods, 2018,10, 3188-3196
  •   Request permissions

    A long-wavelength fluorescent probe for amino compounds and its application in the determination of aliphatic amines

    L. Xia, X. Guo, Y. Ji, L. Chen and H. Wang, Anal. Methods, 2018, 10, 3188
    DOI: 10.1039/C8AY00706C

Search articles by author

Spotlight

Advertisements