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Aptameric detection of Quinine in reclaimed wastewater by using a personal glucose meter

Abstract

The quantification of small molecule analyte in water environment remains expensive due to the high costs of instruments and human resources. Aptamer-invertase biosensor coupled with a personal glucose meter (PGM) is a precise and promising method to substitute the traditional instruments. Here using quinine as an example, we demonstrated the feasibility of using aptamer-invertase biosensor to quantify quinine in reclaimed wastewater. The analytical procedure consisted of building the structure of aptamer-DNA-invertase on magnetic beads, competition between quinine and inverstase, catalyzing sucrose by released invertase, and quantifying glucose by a PGM. The dissociation coefficient of aptamer and quinine was estimated to be 0.36 μM by an isothermal titration calorimetry, indicating very strong affinity between them. The linear calibration curve of quinine was in the range from 0 to 2 μM. The limit of detection (LOD) was estimated to be 0.13 μM for quinine in pure water and 0.32 μM in reclaimed wastewater. The selectivity of quinine detection from other pharmaceutics was satisfied. The aptamer-invertase-PGM method is an inexpensive, affordable and feasible choice for quinine analysis in water management.

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Publication details

The article was received on 18 Mar 2018, accepted on 15 May 2018 and first published on 17 May 2018


Article type: Paper
DOI: 10.1039/C8AY00585K
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Aptameric detection of Quinine in reclaimed wastewater by using a personal glucose meter

    Y. Qiu, C. Gu, B. Li and H. Shi, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00585K

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