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Issue 13, 2018
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A simple device for rapid quantification of cell number from equine buccal swab samples

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Abstract

Biological samples are notoriously difficult to use within portable miniaturised devices, often due to complex sample matrices or variation between cellular samples. Herein we describe a device that utilises a correction based upon the total soluble cellular protein and absorbance measurements for the accurate quantification of cell number from equine buccal swabs. Hemocytometer cell counts were initially conducted to determine average mammalian and bacterial contributions (97.66% and 2.34% respectively). Secondly, cells were lysed and centrifuged before the supernatant was utilised within a Bradford's ratio metric absorbance assay using a microplate reader. A correlation coefficient of r = 0.992 was determined, indicating this method was successful in utilising total soluble cellular protein for the quantification of cell number in unknown biological samples of this type. Upon the identification of a strong linear correlation coefficient, a portable microfluidic device was developed (r = 0.983) as a highly specific platform for real time buccal cell number quantification with the ability to be used for sample correction for buccal swab samples as low as 7.28 μg mL−1 in economic downstream biological diagnostic devices.

Graphical abstract: A simple device for rapid quantification of cell number from equine buccal swab samples

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Publication details

The article was received on 13 Feb 2018, accepted on 23 Feb 2018 and first published on 26 Feb 2018


Article type: Paper
DOI: 10.1039/C8AY00338F
Citation: Anal. Methods, 2018,10, 1523-1528
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    A simple device for rapid quantification of cell number from equine buccal swab samples

    K. Quayle, E. H. Doeven, R. Alexander, Giorgio M. De Guzman, A. F. Clarke, S. J. Haswell, P. S. Francis and X. A. Conlan, Anal. Methods, 2018, 10, 1523
    DOI: 10.1039/C8AY00338F

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