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Rapid determination of histamine in fish by thin-layer chromatography-image analysis method using diazotized visualization reagent prepared with p-nitroaniline

Abstract

A novel analytical method for rapid determination of histamine in fish consists of methanol extraction, manual spotting, thin-layer chromatograph (TLC) for less than 15 min, visualization by diazotized p-nitroaniline reagent, quantitation by image analysis with ImageJ software. Histamine can be successfully detected and completely separated from other biogenic amines and diazonium reagent’s positive compounds. Moreover, the method does not require expensive reagent and instrument as well as any tedious sample pre-treatment. The characterization of this method revealed good specificity, linearity in the range of 100–1200 ng/spot (Adjusted R-square = 0.9987), precision (relative standard deviation 1.07%–2.76%), accuracy (with a trueness of 1.76%–6.22%), recovery rate (93.78%–98.24%), limit of detection (23.49 ng/spot), and limit of quantitation (78.29 ng/spot). The method is simpler, faster and less expensive than HPLC and previously reported TLC/HPTLC. Therefore, the method has the potential to be adapted by aquatic enterprises and aquatic market supervision commissions for monitoring the quality of seafood products during processing, storage, and sales.

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Publication details

The article was received on 12 Feb 2018, accepted on 01 Jun 2018 and first published on 13 Jun 2018


Article type: Paper
DOI: 10.1039/C8AY00336J
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Rapid determination of histamine in fish by thin-layer chromatography-image analysis method using diazotized visualization reagent prepared with p-nitroaniline

    H. Yu, D. Zhuang, X. Hu, S. Zhang, Z. HE, M. Zeng, X. Fang, J. Chen and X. Chen, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00336J

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