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Detection of pulmonary surfactant protein A by using aptamer-based liquid crystal biosensor

Abstract

This paper reports a novel detection method for pulmonary surfactant protein A (SP-A) using aptamer-based liquid crystal (LC) biosensor. The LC molecules are supported on the surface of glass slide modified with an appropriate amount of SP-A aptamers, causing a homeotropic orientation of LCs. The specific interaction between the aptamer and SP-A induces great changes of the topology on the surface. The changes will disrupt the homeotropic orientation of LCs, resulting in an optical appearance transition of polarized image. Therefore, SP-A can be easily recognized. The results can be quantitative determined through the image analysis. The limit of detection is only 5 nM. The presented strategy provides a simple aptamer-based sensing platform for biomolecule recognization, owing to its characteristics of label-free, low cost, high specificity and without requirement of instrumental read-out.

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Publication details

The article was received on 10 Feb 2018, accepted on 10 May 2018 and first published on 11 May 2018


Article type: Paper
DOI: 10.1039/C8AY00326B
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Detection of pulmonary surfactant protein A by using aptamer-based liquid crystal biosensor

    Y. Wang, B. Wang, Q. Zhang, X. Xiong and S. Deng, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00326B

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