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Telomerase Activity Detection in Cancer Cell via Primer Extension-Mediated Fluorescence Enhancement of Silver Nanoclusters

Abstract

Telomerase is a potentially sensitive biomarker for early cancer diagnosis and prognosis, the development of effective methods for telomerase activity detection is important and desirable. In this work, inspired by the tunable fluorescence property of DNA-templated AgNCs which can be enhanced by guanine-rich DNA sequence in proximity, a new fluorescent strategy for telomerase activity detection was proposed. When in the presence of active telomerase which was extracted from cancer cells, repeats of guanine-rich DNA sequence (TTAGGG) would be generated by telomerase-catalyzed extension of the primer, and fluorescence enhancement of AgNCs would be triggered by the hybridization of the extended product with the template DNA of AgNCs. Results demonstrated that the proposed method was sensitive to telomerase activity, could discriminate cancer cell from normal cell, held satisfactory potential for cancer diagnosis and could be applied for telomerase inhibition assay. Thus, we thought that this developed method was meaningful in wide fields including biochemical sensing, molecular biology and biomedicine.

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Publication details

The article was received on 10 Feb 2018, accepted on 27 Mar 2018 and first published on 29 Mar 2018


Article type: Paper
DOI: 10.1039/C8AY00323H
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Telomerase Activity Detection in Cancer Cell via Primer Extension-Mediated Fluorescence Enhancement of Silver Nanoclusters

    X. Li, W. Wang, D. Yang, J. Wu, B. Zhang, Z. Wu and J. Tang, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00323H

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