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Issue 16, 2018
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A sensitive fluorescence method for the detection of streptavidin based on target-induced DNA machine amplification

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Abstract

Recently, small molecule and targeted protein interactions have been applied frequently in quantitative detection, with the streptavidin (SA)–biotin model attracting most attention. We address a novel SA detection method based on the terminal protection of a small-molecule-linked DNA probe from degradation by exonuclease I and G-quadruplex-thioflavin T complex fluorescence signal amplification. With the signal amplification strategy, a limit of detection as low as 0.065 nM was reached. The developed strategy provides a new route to a low cost and highly sensitive and selective method of detection of SA in real samples.

Graphical abstract: A sensitive fluorescence method for the detection of streptavidin based on target-induced DNA machine amplification

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Publication details

The article was received on 06 Feb 2018, accepted on 27 Mar 2018 and first published on 27 Mar 2018


Article type: Paper
DOI: 10.1039/C8AY00293B
Citation: Anal. Methods, 2018,10, 1870-1874
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    A sensitive fluorescence method for the detection of streptavidin based on target-induced DNA machine amplification

    M. Chen, Z. Deng, C. Ma, H. Zhao, K. Wu and K. Wang, Anal. Methods, 2018, 10, 1870
    DOI: 10.1039/C8AY00293B

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