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New and simple electroanalytical method useful for detecting thiomersal in vaccines on a screen-printed electrode modified with chitosan

Abstract

The use of a screen-printed electrode modified with chitosan (Cs) to determine thiomersal (TMS) through the oxidation of the thiosalicylic acid by linear sweep anodic stripping voltammetry is reported for the first time in this work. Screen-printed electrode was coated with chitosan (Cs/SPCE) and the surface was studied with cyclic voltammetry and electrochemical impedance spectroscopy. The modified electrode presented activity towards the oxidation of thiomersal (TMS) at 0.24 V. The signal is product of the oxidation of the thiosalicylic acid or organic part and not of the mercury in the chemical structure of the thiomersal. Optimal parameters (pH 3.2 in phosphate buffer solution-PBS), linear sweep anodic stripping voltammetry with accumulation time; tACC 90s, accumulation potential; EACC -1.0 V and scan rate of 100.0 mV s-1 oxidation signal was proportional to the concentration of TMS between 0.99 and 10.90 µmol L-1, with detection limit of 0.038 µmol L-1 and relative standard deviation (R.S.D) was 5.0 % using seven different electrodes. The sensor was used in human urine samples spiked with thiomersal and commercial cattle vaccines with consistent results.

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Publication details

The article was accepted on 10 Feb 2018 and first published on 12 Feb 2018


Article type: Paper
DOI: 10.1039/C8AY00161H
Citation: Anal. Methods, 2018, Accepted Manuscript
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    New and simple electroanalytical method useful for detecting thiomersal in vaccines on a screen-printed electrode modified with chitosan

    E. Nagles, C. Gonzales and O. García-Beltrán, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00161H

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