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A Fast, Direct and Sensitive Analysis Method for Catechins Determination in Green Tea by Batch Injection Analysis with Multiple-Pulse Amperometry (BIA-MPA)

Abstract

Bioactive compounds have several biological activities promoting health. Among these compounds, those with antioxidants properties, such as vitamins and phenolic compounds, have attracted great interest by their proven effects on protection against oxidative stress. Most of the bioactive phenolic compounds possess high difficult determination by voltammetric techniques at bare electrodes, due to the polymerization process formed on the electrode surface during phenol oxidation. This work describes the determination of bioactive compound catechin by electrochemical oxidation using batch injection analysis (BIA) with multiple amperometric detection (MPA), employing an unmodified glassy carbon (GC) electrode in acetate buffer solution (pH 4.5). The proposed BIA method is based on the application of multiple pulse potentials of 0.4 V (oxidation potential), 1.4 V (cleaning potential), and 0.0 V (conditioning potential) vs. Ag/AgCl (KCl 3 M), hence avoiding passivation by oxidation products and enabling the quantification of catechin. Under these conditions, the repeatability of the method for successive injections of catechin at 50 μmol L−1 (n = 30) was 0.85% and the sampling rate was 120 injections per hour. The amperometric response presented a linear range for solutions containing catechin at 20 to 100 μmol L−1 (R = 0.999), with a detection limit of 0.03 μmol L−1 and a quantification limit of 0.10 μmol L−1. In addition, the proposed method was shown to be fast and sensitive for determination of catechin in real samples and the results are comparable to those obtained with high performance liquid chromatography (HPLC), at a 95% confidence level.

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Publication details

The article was received on 12 Jan 2018, accepted on 03 Apr 2018 and first published on 04 Apr 2018


Article type: Paper
DOI: 10.1039/C8AY00077H
Citation: Anal. Methods, 2018, Accepted Manuscript
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    A Fast, Direct and Sensitive Analysis Method for Catechins Determination in Green Tea by Batch Injection Analysis with Multiple-Pulse Amperometry (BIA-MPA)

    G. Ribeiro, C. Rocha, A. Tanaka and I. S. D. Silva, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00077H

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