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Sulfonated chitooligosaccharide modified silica material for hydrophilic interaction liquid chromatography and its chromatographic evaluation

Abstract

A novel stationary phase derived from sulfonated chitooligosaccharide (COS) was prepared and evaluated in hydrophilic interaction liquid chromatography (HILIC) mode. Fourier-transform infrared spectroscopy (FT-IR), elemental analysis (EA), and thermogravimetry analysis (TGA) supported the successful immobilization of the obtained material. The effects of various parameters including composition of mobile phase, buffer concentration, buffer pH, column temperature, and stability on the retention behavior of the stationary phase were investigated by use of nucleosides and bases. Two empirical models, partitioning and adsorption, had been used to describe the separation mechanism of the stationary phase. The corresponding column could also successfully separate other polar and hydrophilic compounds such as water-soluble vitamins, salicylic acid related compounds, and melamine. The newly synthesized stationary phase displayed the potential application for HILIC mode separation.

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Publication details

The article was received on 12 Jan 2018, accepted on 12 Feb 2018 and first published on 12 Feb 2018


Article type: Paper
DOI: 10.1039/C8AY00070K
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Sulfonated chitooligosaccharide modified silica material for hydrophilic interaction liquid chromatography and its chromatographic evaluation

    K. Yan, H. Yang, S. Huang and Z. Bai, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00070K

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