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Fast simultaneous detection of three diterpenoids in Herba Siegesbeckiae using solid phase extraction followed by HPLC-UV with core–shell particle column

Abstract

To determine the kirenol, darutoside and darutigenol in Herba Siegesbeckiae within HPLC quickly, the ability of sample cleanup with different solid phase extraction was systematically investigated. The results showed that the polymer based strong anion exchange (P-SAX, 200mg/6mL) sorbent could separate and enrich diterpenoids providing rapid determination without impurity interference. In this paper a new HPLC-UV method using core-shell column (Boltimate C18 3.0×100mm, 2.7μm) for the quantitative determination of three diterpenoids was developed. Under the optimum experimental conditions, P-SAX pretreatment with core-shell analytical column exhibited satisfactory results in the determination of diterpenoids in Herba Siegesbeckiae with recovery rate of 95–102%, and relative standard deviations of below 1.1%, and limit of quantitation as low as 0.1 6μg/mL, and short analytical runtime of 8min. At last, the proposed method was successfully applied in the profiling of diterpenoids in different regions of Herba Siegesbeckiae.

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Publication details

The article was received on 09 Jan 2018, accepted on 08 Feb 2018 and first published on 12 Feb 2018


Article type: Paper
DOI: 10.1039/C8AY00046H
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Fast simultaneous detection of three diterpenoids in Herba Siegesbeckiae using solid phase extraction followed by HPLC-UV with core–shell particle column

    Y. Zhang, Z. Zou and G. Chou, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C8AY00046H

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