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Paper based lateral flow immunoassay for the enumeration of Escherichia coli in urine

Abstract

In this experimental study, we developed lateral flow immunoassay strips for detection of Escherichia coli. For labelling, gold nanoparticles were prepared and modified with a Raman label. Meanwhile, not only colorimetric responses but also Raman responses were obtained from the same strips. Color intensities and Raman intensities were employed for quantitative determination of Escherichia coli. Employment of two different detection methods strengthen the quantitative determination of Escherichia coli by lateral flow immunoassay which is mostly used for qualitative detection or sometimes for semi-quantitative determination of samples. After careful optimization and validation steps, the developed strips yielded highly sensitive determination of Escherichia coli in water and urine samples.

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Publication details

The article was received on 27 Dec 2017, accepted on 10 Feb 2018 and first published on 12 Feb 2018


Article type: Paper
DOI: 10.1039/C7AY02974H
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Paper based lateral flow immunoassay for the enumeration of Escherichia coli in urine

    A. Gumustas, M. G. Caglayan, M. Eryilmaz, Z. Suludere, E. Acar Soykut, B. Uslu, I. H. Boyacı and U. Tamer, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C7AY02974H

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