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Electrochemiluminescence biosensing platform for Hg2+ determination based on host-guest interaction between β-cyclodextrin functionalized Pd nanoparticles and ferrocene

Abstract

A solid-state electrochemiluminescence (ECL) switch biosensor using Ru(bpy)32+/β-cyclodextrin-Pd nanoparticles (β-CY-PdNPs)/Gelatin (Gel) complex and ferrocene-labeled DNA probe (Fer-DNA) for the detection of Hg2+ was successfully developed. The ECL biosensor includes an ECL substrate and an ECL signal switch. Ru(bpy)32+/β-CY-PdNPs/Gel composite modified on the glassy carbon electrode was used as an ECL substrate, which could bring about a clear and stable ECL signal by Ru(bpy)32+. Meantime, hairpin-like Fer-DNA probe was acted as an ECL signal switch, which was designed by molecular recognition strategy and attached to β-CY-PdNPs through host-guest interaction between β-CY and ferrocene. Our investigation indicated that, when Hg2+ was absent, the Fer-DNA probe retained its hairpin structure and led to an obvious quenching effect of Ru(bpy)32+ signal. However, when the biosensor incubated with Hg2+, the specific T-Hg2+-T interaction brought about the change of the of Fer-DNA conformation, and such conformation adjustment resulted in a clear signal recovery of Ru(bpy)32+ owing to the reduced quenching effect of ferrocene. The ECL switch biosensor offered good linear responses for Hg2+ in the range of 0.003 ~ 600 ng/mL with a detection limit of 0.0015 ng/mL at the 3sblank level.

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Publication details

The article was received on 22 Dec 2017, accepted on 09 Jan 2018 and first published on 10 Jan 2018


Article type: Paper
DOI: 10.1039/C7AY02944F
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Electrochemiluminescence biosensing platform for Hg2+ determination based on host-guest interaction between β-cyclodextrin functionalized Pd nanoparticles and ferrocene

    Y. Hu, Z. Liu, H. Zhan and Z. Shen, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C7AY02944F

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