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Issue 7, 2018
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An electrochemiluminescence biosensing platform for Hg2+ determination based on host–guest interactions between β-cyclodextrin functionalized Pd nanoparticles and ferrocene

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Abstract

A solid-state electrochemiluminescence (ECL) switch biosensor using a Ru(bpy)32+/β-cyclodextrin–Pd nanoparticles (β-CY–PdNPs)/gelatin (Gel) complex and a ferrocene-labeled DNA probe (Fer-DNA) for the detection of Hg2+ was successfully developed. The ECL biosensor includes an ECL substrate and an ECL signal switch. The Ru(bpy)32+/β-CY–PdNPs/Gel composite modified on a glassy carbon electrode was used as the ECL substrate, which could bring about a clear and stable ECL signal by Ru(bpy)32+. Meantime, the hairpin-like Fer-DNA probe acted as the ECL signal switch, which was designed by a molecular recognition strategy and attached to β-CY–PdNPs through host–guest interactions between β-CY and ferrocene. Our investigation indicated that, when Hg2+ was absent, the Fer-DNA probe retained its hairpin structure and led to an obvious quenching effect of the Ru(bpy)32+ signal. However, when the biosensor was incubated with Hg2+, the specific T–Hg2+–T interaction brought about a change of the Fer-DNA conformation, and such conformation adjustment resulted in a clear signal recovery of Ru(bpy)32+ owing to the reduced quenching effect of ferrocene. The ECL switch biosensor offered good linear responses for Hg2+ in the range of 0.003–600 ng mL−1 with a detection limit of 0.0015 ng mL−1 at the 3sblank level.

Graphical abstract: An electrochemiluminescence biosensing platform for Hg2+ determination based on host–guest interactions between β-cyclodextrin functionalized Pd nanoparticles and ferrocene

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Publication details

The article was received on 22 Dec 2017, accepted on 09 Jan 2018 and first published on 10 Jan 2018


Article type: Paper
DOI: 10.1039/C7AY02944F
Citation: Anal. Methods, 2018,10, 767-774
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    An electrochemiluminescence biosensing platform for Hg2+ determination based on host–guest interactions between β-cyclodextrin functionalized Pd nanoparticles and ferrocene

    Y. Hu, Z. Liu, H. Zhan and Z. Shen, Anal. Methods, 2018, 10, 767
    DOI: 10.1039/C7AY02944F

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