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LC-MS/MS based quantitation of ciprofloxacin and its application to antimicrobial resistance study in Balb/c mouse plasma, urine, bladder and kidneys

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Abstract

The authors proposed a simple, high throughput liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the determination of ciprofloxacin in small sample volumes of plasma, urine, bladder and kidneys of mice. d8-Ciprofloxacin was used as an internal standard (IS) which efficiently tracked the matrix effect in different matrices. The method employed 20 μL of plasma/30 μL of urine or tissue homogenate for sample processing using a simple protein precipitation technique. The processed samples were chromatographed on a C18 column by using a mixture of 5 mM ammonium formate (0.1% formic acid)–acetonitrile as the mobile phase at a flow rate of 0.6 mL min−1 with a gradient elution for 3 min. The calibration curve obtained was linear over the concentration range of 100–5000 ng mL−1 with r2 ≥ 0.99 in all matrices. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. The multiple reaction-monitoring mode (MRM) was used for the quantification of ion transitions at m/z 332.1/230.8 and 340.1/296.1 for the analyte and the IS, respectively. The validated method was used for preclinical studies of antimicrobial resistance in mice.

Graphical abstract: LC-MS/MS based quantitation of ciprofloxacin and its application to antimicrobial resistance study in Balb/c mouse plasma, urine, bladder and kidneys

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Publication details

The article was received on 19 Dec 2017, accepted on 28 Jan 2018 and first published on 29 Jan 2018


Article type: Paper
DOI: 10.1039/C7AY02923C
Citation: Anal. Methods, 2018, Advance Article
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    LC-MS/MS based quantitation of ciprofloxacin and its application to antimicrobial resistance study in Balb/c mouse plasma, urine, bladder and kidneys

    M. K. Matta, A. Chockalingam, A. Gandhi, S. Stewart, L. Xu, K. Shea, V. Patel and R. Rouse, Anal. Methods, 2018, Advance Article , DOI: 10.1039/C7AY02923C

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