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Engineering Oligonucleotide-Based Peroxidase Mimetics for Colorimetric Assay of S1 Nuclease

Abstract

Herein we propose a rational design to construct peroxidase mimetics in response to S1 nuclease by employing guanine-rich oligonucleotides and Cu2+ ions. The enzymatic activities of DNA-Cu(II) are highly dependent upon the binding stoichiometry between Cu2+ and DNA. In the H2O2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine, the catalytic activity of Cu2+ can be accelerated by approximately 88-times in the presence of AG3(T2AG3)3, whereas it shows slight enhancement in the presence of mononucleotide (GMP). The coordination of Cu2+ within DNA structures greatly strengthens its ability to generate reactive oxygen species. The most intriguing finding in this study is that DNA-Cu(II) complexes lose their enzymatic activities after the cleavage of DNA scaffolds by S1 nuclease. A colorimetric method was established for selectively evaluating S1 nuclease activity with the limit of detection of 4.7×10-3 U/mL, only by using label-free oligonucleotides and copper ions without complicated synthesis and apparatus. This facile method can also be applicable for quantitative analysis in biological fluids.

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Publication details

The article was received on 08 Dec 2017, accepted on 12 Feb 2018 and first published on 13 Feb 2018


Article type: Paper
DOI: 10.1039/C7AY02830J
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Engineering Oligonucleotide-Based Peroxidase Mimetics for Colorimetric Assay of S1 Nuclease

    C. He, J. Zhang, W. Li and Y. Fu, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C7AY02830J

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