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A simple and high throughput parallel dual immunoaffinity liquid chromatography-mass spectrometry system for urine drug testing

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Abstract

A simple and rapid method for direct quantitation of drugs in human urine samples was developed using a system composed of an automatic column switch and two home-made capillary immunoaffinity columns (CIACs, 100 μm × 15 cm). Before being injected by an autosampler into the system, the urine sample was filtered through a 0.22 μm membrane. The alternating column regeneration was achieved by using two identical CIACs. One column underwent analyte elution while the other column underwent column regeneration simultaneously. 6-Acetylmorphine (6AM) and oxycodone (OCOD) were studied as examples of opiates in urine. Linear calibration curves were obtained in the concentration ranges of 40–200 ng mL−1 and 50–400 ng mL−1, with a limit of quantitation of 22 ng mL−1 and 47 ng mL−1 for 6AM and OCOD, respectively. The results of this method exhibited good inter-day accuracy and precision. Furthermore, the present method was successfully applied to the determination of 6AM in urine samples of drug abusers.

Graphical abstract: A simple and high throughput parallel dual immunoaffinity liquid chromatography-mass spectrometry system for urine drug testing

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Publication details

The article was received on 28 Nov 2017, accepted on 21 Jan 2018 and first published on 22 Jan 2018


Article type: Communication
DOI: 10.1039/C7AY02755A
Citation: Anal. Methods, 2018, Advance Article
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    A simple and high throughput parallel dual immunoaffinity liquid chromatography-mass spectrometry system for urine drug testing

    T. Chou, C. Wang, A. C. Lua and H. Yang, Anal. Methods, 2018, Advance Article , DOI: 10.1039/C7AY02755A

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