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Issue 12, 2018
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Chemometrics-assisted liquid chromatography-full scan mass spectrometry for simultaneous determination of multi-class estrogens in infant milk powder

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Abstract

In the present study, a smart analytical strategy that combines liquid chromatography-full scan mass spectrometry with the second-order calibration method based on the alternating trilinear decomposition (ATLD) algorithm was developed for the simultaneous determination of seven estrogens in infant milk powders. The seven estrogens were rapidly eluted out within 7.0 min under a simple gradient condition and then, they were detected by mass spectrometry operated in the full scan mode. With the aid of the prominent “second-order advantage” of the algorithm, specific qualitative and quantitative information about the target analytes could be extracted from the complex system even in the presence of considerable peak overlaps, baseline drifts and unknown interferences. The proposed strategy avoided time-consuming and laborious sample pretreatment procedures, resulting in minimal loss of analytes, which improved the analytical accuracy. Average recoveries of the seven estrogens in two spiked infant milk powder samples were in the range of 91.2–104.2% with the relative standard deviations (RSDs) lower than 5.0% (with the exception for 17α-estradiol), and the limits of detection (LOD) ranged from 0.07 to 2.49 ng mL−1. Besides, to further confirm the feasibility and reliability of the proposed method, the same batch of samples was analyzed using the LC-MS/MS method, and the statistical tests showed that no significant difference existed between the two methods, which fully indicated that the proposed strategy could provide satisfactory prediction results in real infant milk powder samples as well as other actual chemical systems.

Graphical abstract: Chemometrics-assisted liquid chromatography-full scan mass spectrometry for simultaneous determination of multi-class estrogens in infant milk powder

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Publication details

The article was received on 25 Nov 2017, accepted on 20 Feb 2018 and first published on 23 Feb 2018


Article type: Paper
DOI: 10.1039/C7AY02733H
Citation: Anal. Methods, 2018,10, 1459-1471
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    Chemometrics-assisted liquid chromatography-full scan mass spectrometry for simultaneous determination of multi-class estrogens in infant milk powder

    X. Sun, H. Wu, Z. Liu, L. Xie, Y. Hu, H. Fang, T. Wang, R. Xiao, Y. Ding and R. Yu, Anal. Methods, 2018, 10, 1459
    DOI: 10.1039/C7AY02733H

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