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Ratiometric detection of biothiols by the DNA-templated silver nanoclusters-Hg2+ system

Abstract

A novel fluorescent sensor for ratiometric detection of biothiols, such as cysteine (Cys) and glutathione (GSH), was developed. This sensor was based on the system composed of fluorescent silver nanoclusters stabilized by single-stranded DNA (DNA-Ag NCs) and Hg2+. The Ag NCs we synthesized had two fluorescence emission peaks, red and green. The red peak was strongly quenched by Hg2+ while the green peak showed a concomitant increase in fluorescence intensity in the presence of Hg2+. On the contrary, when biothiols were added to the DNA-Ag NCs-Hg2+ system, there was an increase in the intensity of the red emission peak and a decrease in the intensity of the green emission peak because of the robust Hg-S interaction. By employing the ratio of the DNA-Ag NCs-Hg2+ system’s red to green fluorescence emission intensity as the function of biothiols’ concentration, we obtained satisfactory detection limits and linear relationships of Cys and GSH. Cys and GSH were detected at concentrations as low as 1.59 nM and 2.88 nM, respectively. The capability and potential for practical applications of this method were also demonstrated by detecting biothiols in human serum samples.

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Publication details

The article was received on 01 Nov 2017, accepted on 07 Jan 2018 and first published on 12 Jan 2018


Article type: Paper
DOI: 10.1039/C7AY02564E
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Ratiometric detection of biothiols by the DNA-templated silver nanoclusters-Hg2+ system

    Z. Yan, C. Tian, X. Sun, Y. Wu, D. Li and B. Ye, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C7AY02564E

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