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Preparation of an anti-formoterol monoclonal antibody for indirect competitive ELISA detection of formoterol in urine and pork samples

Abstract

In this study, an enzyme-linked immunosorbent assay (ELISA) was established to detect formoterol (FMT) residue in pork and urine samples. A monoclonal antibody (mAb) against FMT was prepared using hybridoma technology. The developed ELISA method had a standard curve ranging from 0.098 to 6.25 μg/L. The 50% inhibitory concentration (IC50) value and the limit of detection (LOD) were 1.04 μg/L and 0.15 μg/L, respectively. The cross-reactivity (CR) values of 7 compounds were less than 0.01%, but the CR value of clenbuterol was not (CR<0.62%). Urine and pork samples supplemented with different concentrations of FMT were analysed using ELISA. The intra-assay recovery rates and coefficients of variation were 91.5–118% (n=5) and 7.9–14.7% (n=5), respectively. In contrast, the inter-assay recovery rate ranged from 87% to 124% (n=5), and the coefficients of variation were within 6.3–18.6% (n=5), indicating that the assay had high accuracy and precision. Correlation values for urine and pork were higher (R2=0.99 and 0.98, respectively) for the optimized ELISA than for standard LC-MS/MS analysis in a variety of samples. This optimized mAb-based ELISA provides a rapid method for detecting FMT residue in urine and pork samples with high specificity and sensitivity.

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Publication details

The article was received on 03 Nov 2017, accepted on 26 Dec 2017 and first published on 02 Jan 2018


Article type: Paper
DOI: 10.1039/C7AY02562A
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Preparation of an anti-formoterol monoclonal antibody for indirect competitive ELISA detection of formoterol in urine and pork samples

    C. Li, Y. Li, Y. Zhang, J. Liu, J. Li, M. Wu, H. Liu and Z. Yang, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C7AY02562A

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