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Simultaneous determination of tianeptine and its active metabolite tianeptine MC5 in rat plasma and brain tissue using high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)

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Abstract

A selective and sensitive method was developed to simultaneously quantify tianeptine and its main active metabolite tianeptine MC5 in rat plasma and brain tissue. A one-step liquid–liquid extraction was developed for sample preparation. The linear range for plasma was from 1.0–500.0 ng mL−1 and for brain tissue it was from 1.0–500.0 ng g−1. The method was validated in accordance with US FDA guidelines (precision within 15% relative standard deviation (RSD) and accuracy within 15% relative error (RE) with a consistent recovery). Stable isotope-labeled tianeptine and tianeptine MC5 (tianeptine-D4 and tianeptine MC5-D4) were used as the internal standards achieving good reproducibility while reducing the matrix effects. This method was successfully applied to a preclinical study for the simultaneous determination of tianeptine and tianeptine MC5 in rat plasma and brain tissue.

Graphical abstract: Simultaneous determination of tianeptine and its active metabolite tianeptine MC5 in rat plasma and brain tissue using high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)

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Publication details

The article was received on 24 Oct 2017, accepted on 15 Dec 2017 and first published on 18 Dec 2017


Article type: Paper
DOI: 10.1039/C7AY02506H
Citation: Anal. Methods, 2018, Advance Article
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    Simultaneous determination of tianeptine and its active metabolite tianeptine MC5 in rat plasma and brain tissue using high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)

    N. M. El Zahar, N. Magdy, A. M. El-Kosasy, W. D. Beck, P. M. Callahan, A. V. Terry and M. G. Bartlett, Anal. Methods, 2018, Advance Article , DOI: 10.1039/C7AY02506H

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