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Issue 19, 2018
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Sensitive determination of L-hydroxyproline in dairy products by capillary electrophoresis with in-capillary optical fiber light-emitting diode-induced fluorescence detection

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Abstract

Hydrolyzed leather proteins have been used illegally by some dairy industries in dairy products to increase the protein content in the last few years. As a specific amino acid in collagen, the detection of L-hydroxyproline (L-Hyp) can be used to indicate whether the hydrolyzed leather proteins are added to dairy products or not. A sensitive capillary electrophoretic method with in-capillary optical fiber light-emitting diode-induced fluorescence detection (ICOF-LED-IFD-CE) was developed for the determination of L-Hyp and further for identification of hydrolyzed leather proteins in dairy products. Fluorescein isothiocyanate (FITC) labelled L-Hyp (FITC-Hyp) was detected in a background electrolyte of 20 mM sodium borate at pH 9.4 and an applied voltage of 17 kV in less than 8 min. The limit of detection (LOD) for FITC-Hyp was 0.61 nM at the signal to noise ratio (S/N) of 3. The intraday precision (n = 6) of migration time and peak area was 2.65% and 1.97%, while the interday precision (n = 6) of migration time and peak area was 1.54% and 2.36%, respectively. The accuracy of the proposed method was judged by standard addition method, spectrophotometry method and commercial LIF-CE, and the results indicated that the proposed method was reliable. The ICOF-LED-IFD-CE method was successfully applied to determine L-Hyp in dairy products.

Graphical abstract: Sensitive determination of l-hydroxyproline in dairy products by capillary electrophoresis with in-capillary optical fiber light-emitting diode-induced fluorescence detection

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Publication details

The article was received on 04 Oct 2017, accepted on 23 Dec 2017 and first published on 26 Dec 2017


Article type: Paper
DOI: 10.1039/C7AY02356A
Citation: Anal. Methods, 2018,10, 2211-2216
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    Sensitive determination of L-hydroxyproline in dairy products by capillary electrophoresis with in-capillary optical fiber light-emitting diode-induced fluorescence detection

    H. Ji, X. Zhang, F. Yang, J. Wang, H. Yuan and D. Xiao, Anal. Methods, 2018, 10, 2211
    DOI: 10.1039/C7AY02356A

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