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Hydrogen-bonding-induced colorimetric detection of melamine based on the peroxidase activity of gelatin-coated cerium oxide nanospheres

Abstract

Herein, we developed a simple and rapid colorimetric assay for detection of melamine using gelatin-coated cerium oxide (Gel-CeO2) nanospheres as peroxidase mimics. Highly monodispersed Gel-CeO2 nanospheres were synthesized through a microwave-assisted hydrothermal process. The Gel-CeO2 nanospheres showed excellent peroxidase activity, which can catalyze the oxidation of ABTS (2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) by H2O2, resulting in formation of blue oxidation products. In the presence of melamine, H2O2 will react with melamine through strong hydrogen-bonding. With the consumption of H2O2, the catalytic reaction was interrupted and the blue ABTS oxidation product solution turned pale. There was a linear relationship between absorbance intensity of ABTS oxidation product and logarithm value of melamine concentration ranging from 50 nM to 5.0 mM. Moreover, the detection limit (S/N=3) was 5.5 nM, which is far below the regulatory level. This method was simple, rapid, sensitive and reliable, suggesting the promising practical usage of this sensing system. Finally, this method was applied to melamine detection in milk and milk powder.

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Publication details

The article was received on 26 Sep 2017, accepted on 09 Jan 2018 and first published on 10 Jan 2018


Article type: Paper
DOI: 10.1039/C7AY02296D
Citation: Anal. Methods, 2018, Accepted Manuscript
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    Hydrogen-bonding-induced colorimetric detection of melamine based on the peroxidase activity of gelatin-coated cerium oxide nanospheres

    X. Jin, W. Yin, G. Ni and J. Peng, Anal. Methods, 2018, Accepted Manuscript , DOI: 10.1039/C7AY02296D

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