Jump to main content
Jump to site search


An enzymatic reaction mediated glucose sensor activated by MnO2 nanosheets acting as oxidant and catalyst

Abstract

A self-regulated smart system would be highly desirable for analyte detection, in which a specific environment for detection could be self-modulated and the required reagents could also be in-situ generated without additional addition. Here, we designed an intelligent glucose detection system composed of glucose, glucose oxidase (GOx), MnO2 and 3,3',5,5'-tetramethylbenzidine (TMB), based on the enzymatic oxidation of glucose and dual roles of synthesized MnO2 nanosheets acting as both oxidant and catalyst. Upon the addition of glucose/GOx, the MnO2 nanosheets partially decomposes by the in-situ generated H2O2 via glucose oxidation. Followed by the addition of TMB, a typical color reaction occurs under slightly acidic condition, enabling the colorimetric determination of glucose. For this system, the specific condition and the required reagents for glucose detection can be self-modulated and self-generated via the enzymatic oxidation of glucose to gluconic acid and H2O2, performing smart self-regulated function. This is an outstanding advantage of our designed glucose sensing system. Moreover, the present sensing system responds to glucose quickly and sensitively with a detection limit of 100 nM, and is stable and specific toward glucose detection.

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Apr 2018, accepted on 30 Apr 2018 and first published on 01 May 2018


Article type: Paper
DOI: 10.1039/C8AN00657A
Citation: Analyst, 2018, Accepted Manuscript
  •   Request permissions

    An enzymatic reaction mediated glucose sensor activated by MnO2 nanosheets acting as oxidant and catalyst

    G. Yan, Y. Zhang and W. Di, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00657A

Search articles by author

Spotlight

Advertisements