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In vitro FTIR microspectroscopy analysis of primary Oral Squamous Carcinoma cells treated with cisplatin and 5-fluorouracil: a new spectroscopic approach for studying drug-cell interaction

Abstract

In the present study, human primary Oral Squamous Carcinoma Cells treated with cisplatin and 5-fluorouracil were analyzed, for the first time, by in vitro FTIR Microspectroscopy (FTIRM), to improve the knowledge on the biochemical pathways activated by these two chemotherapics. Up to date, most of the studies regarding FTIRM cellular analysis have been executed on fixed cells from immortalized cell lines. FTIRM analysis performed on primary tumor cells under controlled hydrated conditions provide more reliable information on the biochemical processes occurring in in vivo tumor cells. This spectroscopic analysis allows to get on the same sample and at the same time an overview of the composition and structure of the most remarkable cellular components. In vitro FTIRM analysis of primary Oral Squamous Carcinoma Cells evidenced a time-dependent drug-specific cellular response, also including apoptosis triggering. Furthermore, the univariate and multivariate analysis of IR data evidenced meaningful spectroscopic differences ascribable to alterations affecting cellular proteins, lipids and nucleic acids. These findings suggest for the two drugs different pathways and extent of cellular damage, not provided by conventional cell-based assays (MTT assay and Image-Based Cytometry).

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Publication details

The article was received on 30 Mar 2018, accepted on 28 May 2018 and first published on 30 May 2018


Article type: Paper
DOI: 10.1039/C8AN00602D
Citation: Analyst, 2018, Accepted Manuscript
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    In vitro FTIR microspectroscopy analysis of primary Oral Squamous Carcinoma cells treated with cisplatin and 5-fluorouracil: a new spectroscopic approach for studying drug-cell interaction

    E. Giorgini, S. sabbatini, R. Rocchetti, V. Notarstefano, C. Rubini, C. Conti, G. Orilisi, E. Mitri, D. E. Bedolla and L. Vaccari, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00602D

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