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Ligand-displacement-based two-photon fluorogenic probe for visualizing mercapto biomolecules in live cells, Drosophila brains and zebrafish

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Abstract

Investigating the change in expression level of mercapto biomolecules (GSH/Cys/Hcy) necessitates a rapid detection method for a series of physiological and pathological processes. Herein, we present a ligand-displacement-based two-photon fluorogenic probe based on an Fe(III) complex, TPFeS, which is a GSH/Cys/Hcy rapid detection fluorogenic probe for in vitro analysis and live cell/tissue/in vivo imaging. The “in situ” probe is non-fluorescent and was prepared from a 1 : 2 ratio of Fe(III) and TPS, a novel two-photon (TP) fluorophore with excellent one-photon (OP) and TP properties under physiological conditions, as a fluorescent ligand. This probe shows a rapid and remarkable fluorescence restoration (OFF–ON) property due to the ligand-displacement reaction of mercapto biomolecules in a recyclable manner in vitro. A significant two-photon action cross-section, good selectivity for biothiols, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range allowed the direct visualization of mercapto biomolecules at different levels between normal/drug-treated live cells, as well as in Drosophila brain tissues/zebrafish based on the use of two-photon fluorescence microscopy.

Graphical abstract: Ligand-displacement-based two-photon fluorogenic probe for visualizing mercapto biomolecules in live cells, Drosophila brains and zebrafish

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Publication details

The article was received on 11 Mar 2018, accepted on 28 May 2018 and first published on 05 Jun 2018


Article type: Paper
DOI: 10.1039/C8AN00453F
Citation: Analyst, 2018, Advance Article
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    Ligand-displacement-based two-photon fluorogenic probe for visualizing mercapto biomolecules in live cells, Drosophila brains and zebrafish

    Y. Zhao, Y. Ni, L. Wang, C. Xu, C. Xin, C. Zhang, G. Zhang, X. Xie, L. Li and W. Huang, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00453F

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