Jump to main content
Jump to site search

Issue 9, 2018
Previous Article Next Article

A new functional membrane protein microarray based on tethered phospholipid bilayers

Author affiliations

Abstract

A new prototype of a membrane protein biochip is presented in this article. This biochip was created by the combination of novel technologies of peptide-tethered bilayer lipid membrane (pep-tBLM) formation and solid support micropatterning. Pep-tBLMs integrating a membrane protein were obtained in the form of microarrays on a gold chip. The formation of the microspots was visualized in real-time by surface plasmon resonance imaging (SPRi) and the functionality of a GPCR (CXCR4), reinserted locally into microwells, was assessed by ligand binding studies. In brief, to achieve micropatterning, P19-4H, a 4 histidine-possessing peptide spacer, was spotted inside microwells obtained on polystyrene-coated gold, and Ni-chelating proteoliposomes were injected into the reaction chamber. Proteoliposome binding to the peptide was based on metal–chelate interaction. The peptide-tethered lipid bilayer was finally obtained by addition of a fusogenic peptide (AH peptide) to promote proteoliposome fusion. The CXCR4 pep-tBLM microarray was characterized by surface plasmon resonance imaging (SPRi) throughout the building-up process. This new generation of membrane protein biochip represents a promising method of developing a screening tool for drug discovery.

Graphical abstract: A new functional membrane protein microarray based on tethered phospholipid bilayers

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Feb 2018, accepted on 23 Mar 2018 and first published on 28 Mar 2018


Article type: Paper
DOI: 10.1039/C8AN00260F
Citation: Analyst, 2018,143, 2165-2173
  •   Request permissions

    A new functional membrane protein microarray based on tethered phospholipid bilayers

    M. Chadli, O. Maniti, C. Marquette, B. Tillier, S. Cortès and A. Girard-Egrot, Analyst, 2018, 143, 2165
    DOI: 10.1039/C8AN00260F

Search articles by author

Spotlight

Advertisements