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An improved detection limit and working range of lateral flow assays based on a mathematical model

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Abstract

Lateral flow assays (LFAs) have attracted considerable attention in biomedical diagnostics. However, it's still challenging to achieve a high detection sensitivity and extensive working range, mainly because the underlying mechanism of complex reaction processes in LFAs remains unclear. Many mathematical models have been developed to analyze the complex reaction processes, which are only qualitative with limited guidance for LFA design. Now, a semi-quantitative convection-diffusion-reaction model is developed by considering the kinetics of renaturation of nucleic acids and the model is validated by our experiments. We established a method to convert the LFA design parameters between the simulation and experiment (i.e., inlet reporter particle concentration, initial capture probe concentration, and association rate constant), with which we achieved a semi-quantitative comparison of the detection limit and working range between simulations and experiments. Based on our model, we have improved the detection sensitivity and working range by using high concentrations of the inlet reporter particles and initial capture probe. Besides, we also found that target nucleic acid sequences with a high association rate constant are beneficial to improve the LFA performance. The developed model can predict the detection limit and working range and would be helpful to optimize the design of LFAs.

Graphical abstract: An improved detection limit and working range of lateral flow assays based on a mathematical model

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Publication details

The article was received on 30 Jan 2018, accepted on 15 Apr 2018 and first published on 24 Apr 2018


Article type: Paper
DOI: 10.1039/C8AN00179K
Citation: Analyst, 2018, Advance Article
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    An improved detection limit and working range of lateral flow assays based on a mathematical model

    Z. Liu, Z. Qu, R. Tang, X. He, H. Yang, D. Bai and F. Xu, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00179K

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