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Issue 11, 2018
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Reconstituting donor T cells increase their biomass following hematopoietic stem cell transplantation

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Abstract

In this study, we used a rapid, highly-sensitive, single-cell biomass measurement method, Live Cell Interferometry (LCI), to measure biomass in populations of CD3 + T cells isolated from hematopoietic stem cell transplant (SCT) patients at various times pre- and post-transplant (days 0–100). CD3 + T cell ‘mass spectra’ were obtained from five autologous and 20 allogenic transplant recipients. We found a pronounced rise in median T cell biomass (+25%; p <0.001) shortly after transplant (day 14), which moderated by day 60. Further, the inter-patient and intra-patient cell masses were most variable at days 14 and 30 post-transplant. T cell biomass trends were similar in both autologous and allogenic transplant recipients. These data suggest that T cell biomass changes are associated with immune reconstitution occurring in the first few weeks post-transplant. To our knowledge, this is the first time single-cell biomass measurements have been studied in human clinical trials. With refinement, these data may prove useful in guiding the withdrawal of immunosuppression following SCT, reducing the likelihood of Graft-Versus-Host Disease or cancer relapse occurring.

Graphical abstract: Reconstituting donor T cells increase their biomass following hematopoietic stem cell transplantation

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Publication details

The article was received on 24 Jan 2018, accepted on 15 Mar 2018 and first published on 23 Mar 2018


Article type: Communication
DOI: 10.1039/C8AN00148K
Citation: Analyst, 2018,143, 2479-2485
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    Reconstituting donor T cells increase their biomass following hematopoietic stem cell transplantation

    K. A. Leslie, M. Rasheed, R. T. Sabo, C. C. Roberts, A. A. Toor and J. Reed, Analyst, 2018, 143, 2479
    DOI: 10.1039/C8AN00148K

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