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Issue 8, 2018
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Development and characterization of a scintillating cell imaging dish for radioluminescence microscopy

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Abstract

Radioluminescence microscopy is an emerging modality that can be used to image radionuclide probes with micron-scale resolution. This technique is particularly useful as a way to probe the metabolic behavior of single cells and to screen and characterize radiopharmaceuticals, but the quality of the images is critically dependent on the scintillator material used to image the cells. In this paper, we detail the development of a microscopy dish made of a thin-film scintillating material, Lu2O3:Eu, that could be used as the blueprint for a future consumable product. After developing a simple quality control method based on long-lived alpha and beta sources, we characterize the radioluminescence properties of various thin-film scintillator samples. We find consistent performance for most samples, but also identify a few samples that do not meet the specifications, thus stressing the need for routine quality control prior to biological experiments. In addition, we test and quantify the transparency of the material, and demonstrate that transparency correlates with thickness. Finally, we evaluate the biocompatibility of the material and show that the microscopy dish can produce radioluminescent images of live single cells.

Graphical abstract: Development and characterization of a scintillating cell imaging dish for radioluminescence microscopy

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Publication details

The article was received on 18 Jan 2018, accepted on 07 Mar 2018 and first published on 08 Mar 2018


Article type: Paper
DOI: 10.1039/C8AN00106E
Citation: Analyst, 2018,143, 1862-1869
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    Development and characterization of a scintillating cell imaging dish for radioluminescence microscopy

    D. Sengupta, T. J. Kim, S. Almasi, S. Miller, Z. Marton, V. Nagarkar and G. Pratx, Analyst, 2018, 143, 1862
    DOI: 10.1039/C8AN00106E

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