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Intracellular endogenous glutathione detection and imaging by a simple and sensitive spectroscopic off-on probe

Abstract

Glutathione (GSH) exerts many cellular functions in human pathologies. Sensitive and simple method capable of assaying GSH would be useful means to understand the mechanism of GSH related diseases. In this work, A new colorimetric and fluorescent off-on probe, 3-oxo-3H-phenoxazin-7-ylthiophene-2-carboxylate, is constructed,synthesized and applied to determine the fluctuation of intracellular GSH selectively and sensitively. The latent fluorescent probe was designed by connecting resorufin with thiophenecarboxylate, allowing it to show high sensitivity (LOD 8.9 × 10-7 M) and off-on fluorescent response to GSH over other different physiological species in the pH 7.4 buffer solutions. The new reaction mechanism based on the cut-through of thiophenecarboxylate in the probe by GSH is confirmed via the HPLC (high performance liquid chromatography) and MS (mass spectrometry) analytical methods. Afterwards, the probe was successfully applied to image GSH in A549 cells and indicate the fluctuations under the stimulation of chemical and drug, which is verified by the investigation of the cell lysate with the commonly-used commercial assay kit. As a result, it is feasible to monitor the level of GSH in biosamples.

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Publication details

The article was received on 18 Jan 2018, accepted on 03 Apr 2018 and first published on 13 Apr 2018


Article type: Paper
DOI: 10.1039/C8AN00102B
Citation: Analyst, 2018, Accepted Manuscript
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    Intracellular endogenous glutathione detection and imaging by a simple and sensitive spectroscopic off-on probe

    H. Zhou, J. Tang, L. Lv, N. Sun, J. Zhang, B. Chen, J. Mao, W. Zhang, J. Zhang and J. Zhou, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00102B

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