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Issue 9, 2018
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A simple and sensitive detection of small molecule–protein interactions based on terminal protection-mediated exponential strand displacement amplification

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Abstract

We herein describe a simple and sensitive strategy to detect a small molecule–protein interaction based on terminal protection-mediated exponential strand displacement amplification (eSDA). In principle, the small molecule linked to a DNA probe protects the DNA probe against the exonuclease I-catalyzed degradation after its binding to the corresponding target protein. The protected DNA probe then serves as a template to promote eSDA. Consequently, a large number of duplexes are produced, which leads to a high fluorescence from a double-stranded DNA specific fluorescent dye, SYBR Green I. As a model system to prove this sensing strategy, the interaction between biotin and streptavidin (SA), which is known to be the strongest among the non-covalent biological interactions, was selected and its analytical performance was thoroughly investigated. As a result, SA was sensitively detected with the limit of detection of 16 pM. In addition, the practical applicability of this method was successfully demonstrated by reliably determining the SA in human serum.

Graphical abstract: A simple and sensitive detection of small molecule–protein interactions based on terminal protection-mediated exponential strand displacement amplification

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Publication details

The article was received on 17 Jan 2018, accepted on 15 Mar 2018 and first published on 21 Mar 2018


Article type: Paper
DOI: 10.1039/C8AN00099A
Citation: Analyst, 2018,143, 2023-2028
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    A simple and sensitive detection of small molecule–protein interactions based on terminal protection-mediated exponential strand displacement amplification

    C. Y. Lee, H. Y. Kim, S. Kim, K. S. Park and H. G. Park, Analyst, 2018, 143, 2023
    DOI: 10.1039/C8AN00099A

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