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Issue 6, 2018
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Electrochemical method for the quantitative determination of Escherichia coli based on gold functionalized FTO substrate

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Abstract

This work presents a novel rapid and sensitive label-free electrochemical method for the detection of bacteria on surface nanostructures. A simple electrochemical deposition and calcination method is employed to prepare different gold nanostructures on FTO substrate. The sensor based on nanostructure gold exhibits excellent linear relation between E. coli DH5α bacteria and the changes of ΔRct, especially FTO-GEDC-D30, with a correction coefficient R2 = 0.998. Both the spectrophotometric (OD600 methods) and fluorescence-staining methods also verified the reliability of electrochemical impedance spectroscopy (EIS) methods for evaluating the antibacterial activity of the gold nanostructure.

Graphical abstract: Electrochemical method for the quantitative determination of Escherichia coli based on gold functionalized FTO substrate

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Publication details

The article was received on 10 Jan 2018, accepted on 06 Feb 2018 and first published on 06 Feb 2018


Article type: Paper
DOI: 10.1039/C8AN00048D
Citation: Analyst, 2018,143, 1462-1467
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    Electrochemical method for the quantitative determination of Escherichia coli based on gold functionalized FTO substrate

    P. Li, X. Wang, W. Gao, W. Yan, W. Guan, Y. Ma, Y. Yu, F. Ji, S. Wu and K. Ding, Analyst, 2018, 143, 1462
    DOI: 10.1039/C8AN00048D

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