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Deciphering of biochemical similarities and differences among mouse embryonic stem cells, somatic and cancer cells using ATR-FTIR spectroscopy

Abstract

Cellular macromolecules play important roles in cellular behaviors and biological processes. In the current work, cancer (KLN205), normal (MSFs) and mouse embryonic stem cells (mESCs) were compared using ATR-FTIR spectroscopy. Modifications in the composition, concentration, structure and function-related changes in the cellular components were deciphered in the infrared spectra. Our results revealed that cancer and embryonic stem cells are very similar but highly different from the normal cells based on the spectral variations in the protein, lipid, carbohydrate and nucleic acid components. The longest lipid acyl chains exist in mESCs while cancer cells harbor the lowest lipid amount, the short lipid acyl chains, a high content of branched fatty acids and thin cell membrane. The highest cellular growth rate and accelerated cell divisions were observed in cancer cells. However, normal cells harbor low nucleic acids and glycogen amounts but have a higher population of lipid composition. Any defect in the signaling pathways and/or biosynthesis of these cellular parameters during the embryonic-to-somatic cell transition may lead to physiological and molecular events that promote the cancer initiation, progression and drug resistance. We conclude that an improved understanding of both similarities and differences in the cellular mechanisms among cancer, normal and mESCs is crucial to develop a potential clinical relevance, and ATR-FITR can be successfully used as a novel approach to gain new insights into the stem cell and cancer researches. We suggest that targeting the cellular metabolisms (glycogen and lipid) can provide new strategies for cancer treatment.

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Publication details

The article was received on 04 Jan 2018, accepted on 09 Feb 2018 and first published on 09 Feb 2018


Article type: Paper
DOI: 10.1039/C8AN00017D
Citation: Analyst, 2018, Accepted Manuscript
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    Deciphering of biochemical similarities and differences among mouse embryonic stem cells, somatic and cancer cells using ATR-FTIR spectroscopy

    G. Güler, E. Acikgoz, N. Ü. Karabay Yavasoglu, B. Bakan, E. Goormaghtigh and H. Aktug, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00017D

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