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Issue 7, 2018
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DNA-templated copper nanoparticles for voltammetric analysis of endonuclease activity

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Abstract

DNA endonucleases play critical roles in medicinal chemistry, which are also commonly used in molecular biology investigation. Sensitive quantification of endonuclease activity is of great significance. In this study, a reliable electrochemical approach for endonuclease activity sensing is developed with the adoption of copper nanoparticles (CuNPs) as electrochemical reporters. Firstly, a DNA duplex is designed and modified on a gold electrode, which acts as the template for the synthesis of CuNPs. Subsequently, the formed NPs are dissolved and then electrodeposited on a glassy carbon electrode for DPV measurements. With the effect of target endonuclease, the DNA duplex is specifically recognized and cleaved. Thus, CuNPs cannot be synthesized and a declined DPV peak is obtained to reveal the level of endonuclease activity. This developed sensor has a wide linear range from 10−3 to 10 U mL−1, and the limit of detection is 10−3 U mL−1, which is extremely low. High stability and excellent reproducibility are also researched. Besides, this sensor shows good selectivity, which can successfully distinguish target endonuclease from possible interferences.

Graphical abstract: DNA-templated copper nanoparticles for voltammetric analysis of endonuclease activity

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Publication details

The article was received on 02 Jan 2018, accepted on 17 Feb 2018 and first published on 20 Feb 2018


Article type: Paper
DOI: 10.1039/C8AN00005K
Citation: Analyst, 2018,143, 1685-1690
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    DNA-templated copper nanoparticles for voltammetric analysis of endonuclease activity

    X. Chen, D. Yang, Y. Tang and P. Miao, Analyst, 2018, 143, 1685
    DOI: 10.1039/C8AN00005K

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