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A narrow open tubular column for high efficiency liquid chromatographic separation

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Abstract

We report a great feature of open tubular liquid chromatography when it is run using an extremely narrow (e.g., 2 μm inner diameter) open tubular column: more than 10 million plates per meter can be achieved in less than 10 min and under an elution pressure of ca. 20 bar. The column is coated with octadecylsilane and both isocratic and gradient separations are performed. We reveal a focusing effect that may be used to interpret the efficiency enhancement. We also demonstrate the feasibility of using this technique for separating complex peptide samples. This high-resolution and fast separation technique is promising and can lead to a powerful tool for trace sample analysis.

Graphical abstract: A narrow open tubular column for high efficiency liquid chromatographic separation

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Publication details

The article was received on 21 Dec 2017, accepted on 15 Mar 2018 and first published on 15 Mar 2018


Article type: Communication
DOI: 10.1039/C7AN02065A
Citation: Analyst, 2018, Advance Article
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    A narrow open tubular column for high efficiency liquid chromatographic separation

    H. Chen, Y. Yang, Z. Qiao, P. Xiang, J. Ren, Y. Meng, K. Zhang, J. Juan Lu and S. Liu, Analyst, 2018, Advance Article , DOI: 10.1039/C7AN02065A

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