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A rapid and specific bacterial detection method based on cell-imprinted microplates

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Abstract

Bacterial detection has attracted substantial interest in recent years owing to its importance in biology, medical care, drug discovery, and public health. For such applications, bacterial cell-imprinting technologies are regarded as potential methods, as they can fabricate artificial tailor-made receptors for cellular recognition. In comparison to conventional methods, which generally require a few days for bacterial determination, cell-imprinted polymers can save a substantial amount of time. Here, we report a high-throughput bacterial detection method based on a cell-imprinted 96-well microplate. The fabrication of the bacterial cell-imprinted polypyrrole and nafion complex was accomplished on a gold nanoparticle-coated microplate. The cell-imprinted polymer complex on the microplate can spontaneously rebind and specifically detect target cells with high selectivity in a short time frame (within 30 min). Furthermore, the microplates could discriminate particular target Escherichia coli O157:H7 cells from bacterial mixtures. This simple method may be used for a variety of applications such as clinical testing, food safety, and continuous environmental monitoring.

Graphical abstract: A rapid and specific bacterial detection method based on cell-imprinted microplates

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Publication details

The article was received on 20 Dec 2017, accepted on 19 Jan 2018 and first published on 23 Jan 2018


Article type: Paper
DOI: 10.1039/C7AN02057K
Citation: Analyst, 2018, Advance Article
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    A rapid and specific bacterial detection method based on cell-imprinted microplates

    X. Shan, T. Yamauchi, Y. Yamamoto, H. Shiigi and T. Nagaoka, Analyst, 2018, Advance Article , DOI: 10.1039/C7AN02057K

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