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A smartphone-based double-channel fluorescent device for immunoassay of carcinoembryonic antigen using CuS nanoparticles for signal amplification

Abstract

Sensitive detection of cancer biomarkers is valuable for clinical diagnosis and treatment assessment of cancers. Herein, we report a simple smartphone-based double-channel fluorescent setup for immunoassay. Except the smartphone, the total cost of the detection device itself is about 80 $, including a laser pointer, a twinning measurement cell, a collective lens, and an outside box. The fluorescent images in the sample and reference areas were captured by the camera in smartphone and the brightness ratio was calculated by a user-edited smartphone APP. By using 2, 3-diaminophenazine (DAP) as the model luminophore, the influence of exposure time and photosensibility on the sensitivity was tested. The brightness ratio offers high stability of the fluorescent signal, which is helpful to improve the sensitivity. The applicability of this device was demonstrated by a catalytic fluorimetric method for Cu2+ determination, which is based on the oxidation of o-phenylenediamine (non fluorescent) to DAP (strong fluorescent) by dissolved oxygen using Cu2+ as the catalyst. Accordingly, by using CuS nanoparticles conjugated second antibody as the signal tag, the immunoassay for carcinoembryonic antigen was performed with the detection limit of 0.05 pg/mL. This smartphone-based double-channel fluorescence device offers the advantages of high sensitivity, inexpensive and miniaturization.

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Supplementary files

Publication details

The article was received on 08 Dec 2017, accepted on 10 Feb 2018 and first published on 12 Feb 2018


Article type: Paper
DOI: 10.1039/C7AN01988B
Citation: Analyst, 2018, Accepted Manuscript
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    A smartphone-based double-channel fluorescent device for immunoassay of carcinoembryonic antigen using CuS nanoparticles for signal amplification

    H. Wang, Y. Sun, W. Yue, Q. Kang, H. Li and D. Shen, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C7AN01988B

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